mouse il 1ra il 1f3 quantikine elisa kit  (R&D Systems)

Journal: bioRxiv

Article Title: Chronic NLRP3 inflammasome activation drives neutrophil brain entry and interactions with microglia

doi: 10.64898/2026.04.22.720282

Figure Lengend Snippet: hNLRP3 D305N mice show constitutive inflammasome activity in the brain, broad neuroinflammatory signaling, and signs of BBB permeability and neuronal damage (a) IL-1β MSD and IL-1RA ELISA quantification of bulk brain samples (n = 6-13/group). (b-c) Bulk brain samples were assayed in a cytokine panel. Select cytokines are shown in column graphs (n = 3-13/group) and the full panel is shown as heatmap (columns represent individual mice). Heatmap values are normalized to hNLRP3 WT . (d-e) Representative images and quantification of western blot for IgG heavy chain (HC) and IgG light chain (LC) in bulk brain samples. Vinculin is used as loading control. Normal two-way ANOVA with Tukey multiple testing correction for comparisons across genotype (n = 5-7/group). (f) Nf-L Simoa assay quantification in CSF samples (n = 6-13/group). All panels include hNLRP3 WT , mNlrp3 WT , and hNLRP3 D305N mice. Unless otherwise noted, lognormal two-way ANOVA with Tukey multiple testing correction for comparisons across genotype and age group. *p adj < 0.05, **p adj < 0.01, ***p adj < 0.001. Data are shown as mean ± SD. Full source data and statistic values in Source Data.

Article Snippet: R&D Systems Mouse IL-1ra/IL-1F3 Quantikine ELISA Kit was used to assess IL-1RA levels and MBL Mouse IL-18 ELISA Kit was used to assess IL-18 levels.

Techniques: Activity Assay, Permeability, Enzyme-linked Immunosorbent Assay, Western Blot, Control

Journal: bioRxiv

Article Title: Social isolation of aged mice drives dramatic release of inflammatory lipoxygenase-derived oxylipins

doi: 10.64898/2025.12.15.694286

Figure Lengend Snippet: (a) Body weight of adult (5 months) and aged (20 months) mice after being kept in group-housing (Ad-GH and O-GH, respectively) or repeatedly isolated (O-ISO). (b,c) Concentration of the cytokines IL-1β and IL-1ra in pg per 50 mg organ. (d) Principal component analysis of organ-specific oxylipin profiles. Hexagons indicate the mean PCA score of all screened replicates within respective experimental groups. (e) Log 2 -fold changes for the organ-specific concentrations of individual oxylipins for the comparison of aged (O-GH) and adult (Ad-GH) mice kept in group-housing (left panel), and the comparison of aged, repeatedly isolated (O-ISO) and aged, group-housed (O-GH) mice (right panel). Fold-changes that could not be calculated due to missing values (e.g., below limit of detection = nd) are depicted in grey. Statistics : Data are shown as ( a - c ) mean ± SEM. The number of biological replicates is n = 5 for Ad-GH and O-ISO, n = 6 for O-GH. Unpaired, two-tailed Student’s t -tests with or without Welch-correction were performed for indicated comparisons. #, comparison of O-GH versus Ad-GH.

Article Snippet: Quantification of cytokines in organ homogenates was performed using commercially available ELISA kits for murine IL-1β (DY401-05) and IL-1ra (DY406-15), manufactured by R&D Systems (Minneapolis, MN, USA).

Techniques: Isolation, Concentration Assay, Comparison, Two Tailed Test

Journal: bioRxiv

Article Title: Social isolation of aged mice drives dramatic release of inflammatory lipoxygenase-derived oxylipins

doi: 10.64898/2025.12.15.694286

Figure Lengend Snippet: (a) Body weight of aged (20 month) mice after being repeatedly isolated into single cages with (O-EX) or without running wheels (O-ISO) for 3 separate nights per week over a period of 8 weeks. (b) Total running performance of O-EX mice is summarised in km over the experimental period of eight weeks. (a,b) Numbers indicate the individual animals in the O-EX cohort. (c,d) Concentration of IL-1β and IL-1ra in pg per 50 mg organ from O-ISO and O-EX mice. (e) Principal component analysis of organ-specific oxylipin profiles from O-ISO and O-EX mice. Hexagons indicate the mean PCA score of all screened replicates within respective experimental groups. (f) Log 2 -fold changes for the organ-specific levels of individual oxylipins comparing the levels of O-EX versus O-ISO mice. Fold-changes that could not be calculated due to missing values (e.g., below limit of detection = nd) are depicted in grey. (g,h) Total amounts of grouped oxylipin species derived from the COX or LOX pathways in (g) fat and (h) liver. Oxylipins were grouped as indicated in . Values are given as ng per 50 mg organ. Limit of detection (LOD) of oxylipins is indicated, if applicable. Statistics : Data are shown as ( a-d , g-h ) mean ± SEM. The number of biological replicates is n = 5 for O-ISO and n = 5-6 for O-EX. Unpaired, two-tailed Student’s t -tests with or without Welch-correction were performed for indicated comparisons.

Article Snippet: Quantification of cytokines in organ homogenates was performed using commercially available ELISA kits for murine IL-1β (DY401-05) and IL-1ra (DY406-15), manufactured by R&D Systems (Minneapolis, MN, USA).

Techniques: Isolation, Concentration Assay, Derivative Assay, Two Tailed Test